inclusion body
英 [ɪnˈkluːʒn ˈbɒdi]
美 [ɪnˈkluːʒn ˈbɑːdi]
n. 包涵体
英英释义
noun
- any small intracellular body found within another (characteristic of certain diseases)
- an inclusion in the cytoplasm of the cell
双语例句
- The solubility analysis showed that the recombinant protein existed as inclusion body in the E.
表达方式分析发现该重组蛋白在大肠杆菌中主要以包涵体的形式存在。 - Diagnosis of Polymerase Chain Reaction ( PCR) and Clinical Pathological Study of Chicken Inclusion Body Hepatitis
鸡包涵体肝炎的PCR诊断和临床病理学研究 - The recombinant CFP10 protein was expressed in inclusion body in E.coli BL21 ( DE3), and the target gene had been cloned into host bacterium.
结果构建了具有正确基因序列的CFP10重组表达质粒,重组蛋白在大肠杆菌BL21(DE3)中以包涵体形式表达。 - Methods: Wash inclusion body effectivity, use Hydrophobic Interaction Chromatography to carry on the purification.
方法:对包涵体进行有效洗涤,采用疏水层析进行纯化。 - Inclusion body protein renaturation is a important procedure in the biotechnology.
包涵体蛋白的复性是生物工程下游技术中的一个重要难题。 - Therefore, the SDS-PAGE gel extraction combining with electroosmosis and dialysis recycling are good and easy to purify the inclusion body proteins.
因此,SDS-PAGE切胶纯化后电渗、透析回收是纯化重组包涵体蛋白有效、简便的方法。 - Both pure recombinant protein were obtained by using inclusion body purification, renaturation and cobalt affinity chromatography, respectively.
分别对表达的蛋白进行包涵体纯化、复性研究及钴柱亲和层析纯化。 - The target protein was purified by denaturation and renaturation from inclusion body, and was detected by Western blot.
通过包涵体变复性纯化目的蛋白,并通过westernblot鉴定目的蛋白。 - Result The inclusion body proteins could be partially renatured, its specific activity was about 900U/ mg protein.
结果包涵体蛋白可以部分复性,比活达到900Umgprotein。 - OBJECTIVE: To reduce the formation of inclusion body in the course of fermentation of E.
目的:减少大肠杆菌发酵过程中包含体的形成。
